Calibrated Gradients

In high-throughput purification, even small retention time shifts between analytical and preparative LC systems can lead to missed or incorrect fractions. Virscidian’s Analytical Studio Sample List Builder (AS-SLB) now offers an elegant solution: calibrated gradients.

This new capability automatically maps analytical retention times to the precise solvent conditions required on prep LC systems, thereby ensuring each compound elutes predictably and exactly where expected.

The result: reduced method development, fewer reruns, and higher confidence in every fraction collected.  

This tech note outlines how to implement calibrated gradients in AS-SLB and the measurable impact on purification reliability, throughput, and data quality.