Enabling High Throughput MRM Based Biomarker Validation Studies Through A Vendor Neutral Application

Joseph Simpkins

A modern current trend in biomarker validation is to profile well known systems using specific higher sensitivity assays based on quantitative mass spectrometry MRM experiments.  Chemical biomarkers evaluated using these tools range from multiple peptide targets in plasma to panels of small molecules (e.g. biogenic amines) in cerebral spinal fluid. In all cases the potential biomarkers were quantified based on reference standards with a goal to better understand the response of the patient to therapeutic treatments.  The software tools developed and applied to this study improved the success and throughput of these targeted assay studies.