Automated Method Development for High Throughput Purification at Nurix Therapeutics

Focused gradients are commonly used in purification workflows to quickly develop preparative conditions for a large diversity of samples. To maximize efficiency, it is common to only consider single screening and preparative conditions. At Nurix, we use Analytical Studio Pro (Virscidian, Inc.), a chromatography and mass spectrometry analysis software package, which allows analysts to interpret large data sets. We have customized this software to assist with preparative method selection factoring in multiple stationary phases, mobile phases, sample properties, and requested product specifications. This platform supports singleton and library workflows and has enabled a ten-fold increase in productivity per analyst.